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GF156

Sigma-Aldrich

B18R, Human Recombinant Carrier-Free

liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)

Synonym(s):

B18R protein, Recombinant B18R

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
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biological source

human

Quality Level

100

Assay

>98% (SDS-PAGE)

form

liquid

specific activity

≥1.3 x 10e6 units/mg protein

mol wt

Mw 38,389 Da

concentration

0.5 mg/mL

technique(s)

cell culture | stem cell: suitable

impurities

<0.05 ng/μg cytokine (LAL test)

input

sample type induced pluripotent stem cell(s)

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B18R, Human Recombinant Carrier-Free liquid, &gt;98% (SDS-PAGE), &lt;0.05 ng/&#956;g cytokine (LAL test)

GF156

B18R, Human Recombinant Carrier-Free

白细胞介素-1&#946;蛋白，重组人类 The recombinant human Interleukin-1beta protein (or IL-1beta protein) is a potent immuno-modulator which mediates a wide range of immune &amp; inflammatory responses including the activation of B &amp; T-cells.

IL038

白细胞介素-1β蛋白，重组人类

Interleukin-12 Protein, Recombinant human The recombinant human Interleukin-12 protein (or IL-12 protein) is a potent regulator of cell mediated immune responses &amp; it induces IFN-gamma production by NK &amp; T cells.

IL029

Interleukin-12 Protein, Recombinant human

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GF136

BAFF Protein, Recombinant human

biological source

human

biological source

human

biological source

human

biological source

human

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

concentration

0.5 mg/mL

concentration

-

concentration

-

concentration

-

mol wt

Mw 38,389 Da

mol wt

-

mol wt

-

mol wt

Mw 17.0 kDa

form

liquid

form

-

form

-

form

-

General description

Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.
Research area: Immuno and CKS B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.

Application

B18R, Human RecombinantCarrier-Free has been used as a decoy receptor to study the intrinsic antiviralactivity of recombinant soluble IFNα/β cell surface receptor 2 (sIFNAR2).[1] Ithas also been used in the generation and maintenance of induced pluripotentstem cells (iPSCs) and human IPSCs.[2][3]

Physical form

Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.

Storage and Stability

For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.

Analysis Note

The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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