FCMAB181F
Milli-Mark® Anti-CD14 -FITC Antibody, clone TUK4
clone TUK4, Milli-Mark®, from mouse
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Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
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biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TUK4, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CD14(929)
Related Categories
General description
CD14 is composed of 356 amino acids and is anchored to the cell membrane by linkage to glycosyl phosphatidylinositol. In addition to the 53-55 kDa cell surface form, two soluble forms have been described (Goyert, 2002). CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes (Goyert, 2002). Most tissue macrophages and Langerhans cells also express CD14 (Goyert, 1999 and 2002; Gadd, 1989). Anti-CD14, TUK4, was included in the Fourth, Fifth and Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD14 (Goyert 1995 and 1996).
Specificity
Antibody recognizes Human CD14 antigen.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Milli-Mark Anti-CD14 -FITC Antibody, clone TUK4 is validated for use in FC for the detection of CD14.
Quality
Evaluated by flow cytometry using human PBMCs.
Target description
53-55 kDa (Goyert, 2002)
Physical form
Protein A purified
Purified Mouse monoclonal IgG2a conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Storage and Stability
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Analysis Note
Control
PBMCs
PBMCs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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