Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).

Antibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.

Epitope: Phosphorylated Thr185/Phosphorylated Tyr187

Phosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated

Research Category
Signaling

Research Sub Category
MAP Kinases

This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.

Evaluated by Flow Cytometry with Jurkat cells.

42kDa and 44kDa Observed

Protein A purified

Purified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide

Stable for 6 months at 2-8°C from date of receipt. Protect from light.

Control
Jurkat Cells

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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