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F1904

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, purified 120 kDa α Chymotryptic Fragment (cell attachment region), suitable for cell culture

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UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified,

biological source

human

Quality Level

100

form

lyophilized

mol wt

120 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

NCBI accession no.

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

UniProt accession no.

P02751

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)

Related Categories

General description

PROPERTIES:

Contains the cell attachment region of the fibronectin molecule.
Product Source: Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.

Application

F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

Physical form

Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.

Storage and Stability

Maintain material at -20°C for 3 months or -70°C for six months.

Analysis Note

One major band at 120kDa in SDS-PAGE

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Certificates of Analysis (COA)

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Fibronectin: purification, immunochemical properties, and biological activities.
Ruoslahti, E, et al.
Test, 82 Pt A, 803-831 (1982)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)
Abir Chakraborty et al.
Cells, 9(2) (2020-01-26)
Heat shock protein 90 (HSP90) is an evolutionarily conserved chaperone protein that controls the function and stability of a wide range of cellular client proteins. Fibronectin (FN) is an extracellular client protein of HSP90, and exogenous HSP90 or inhibitors of

Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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