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EZRMFGF21

Millipore

Rat/Mouse FGF-21 ELISA Kit

measures and quantifies FGF-21 levels in 10 µl - 20 µl serum, plasma or cell culture supernatent

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Synonym(s):
Fibroblast growth factor 21
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Rat / Mouse FGF-21 ELISA Kit, This Rat / Mouse FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.

Quality Level

200

species reactivity

mouse, rat

packaging

kit of 1 × 96 wells

parameter

10—20 μL sample volume (4hr assay)

assay range

standard curve range: 50-12000 pg/mL
inter-assay cv: <8%
intra-assay cv: <10%

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Recent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. No significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.

Although considerable progress has been made in the understanding of the pathobiology of type 2 diabetes, as well in the development of new compounds for the treatment of diabetic patients, this disease remains a major global health concern. Consequently, Millipore is pleased to offer the Rat/Mouse FGF-21 ELISA kit for the quantification of FGF-21 in rat and mouse serum and plasma samples. The insulin-independent activities of FGF-21, as well as the absence of the weight gain effects of insulin, thiazolidinediones and other anti-diabetic drugs, distinguish it from current insulin-sensitizing drugs, making it a promising therapeutic.

Specificity

Cross-reactivity to Human FGF-21: 39%

No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.

Application

Research Category
Metabolism
Research Sub Category
Diabetes - Type 2

Metabolic Syndrome
This is a 3-hour assay requiring 20 μL (in duplicate) of rat sample or 10µl (in duplicate) of mouse sample.
Used to detect/quantify: FGF-21

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Certificates of Analysis (COA)

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Induction of ketosis in rats fed low-carbohydrate, high fat diets depends on the relative abundance of dietary fat and protein.
Bielohuby, Maximilian, et al.
American Journal of Physiology. Endocrinology and Metabolism (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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