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EZRGRT

Millipore

Rat/Mouse Ghrelin ELISA Kit

measures and quantifies total Ghrelin in 20 μL serum or plasma

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Synonym(s):
Appetite Regulating Hormone, Growth hormone-releasing peptide, Interleukin-6, Motilin-related peptide
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Rat/Mouse Ghrelin (total) ELISA, This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.

Quality Level

200

species reactivity

mouse, rat

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

assay range

accuracy: 80-103%
(Serum: Active Ghrelin)
accuracy: 86-102%
(Plasma: Active Ghrelin)
accuracy: 92-103%
(Serum: Des-octanoyl Ghrelin)
accuracy: 95-107%
(Plasma: Des-octanoyl Ghrelin)
inter-assay cv: 1.8-4.5%
intra-assay cv: 0.7-1.3%
(Serum)
inter-assay cv: 2.0-3.2%
intra-assay cv: 1.1-1.7%
(Plasma)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

Q9EQX0 (mouse)
Q9QYH7 (rat)
Q9UBU3

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

Specificity

Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%

Application

Research Category
Metabolism
Research Sub Category
Obesity

Metabolic Disorders
Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Used to detect/quantify: Ghrelin (Total)

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dual regulatory role for phosphatase and tensin homolog in specification of intestinal endocrine cell subtypes.
Sebastien Ab Roy,Marie-Josee Langlois,Julie C Carrier,Francois Boudreau,Nathalie Rivard et al.
World Journal of Gastroenterology null
Diet-induced obesity attenuates fasting-induced hyperphagia.
D I Briggs,M B Lemus,E Kua,Z B Andrews
Journal of Neuroendocrinology null
Diet-induced obesity causes ghrelin resistance in arcuate NPY/AgRP neurons.
Briggs DI, Enriori PJ, Lemus MB, Cowley MA, Andrews ZB
Endocrinology null

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