Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

EZMCP1

Sigma-Aldrich

Human MCP-1 ELISA Kit

detects MCP-1 in 50 μL serum, plasma or cell culture supernatent

Synonym(s):

C-C motif chemokine 2, Monocyte chemoattractant protein 1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.84

product name

Human MCP-1 Conferma® ELISA, quantification of human MCP-1 in serum, plasma, or culture media samples.

clone

monoclonal

Quality Level

200

species reactivity

human

parameter

50 μL sample volume (4hr assay)

assay range

linearity: 94-106 (serum)%
linearity: 94-110 (plasma)%
inter-assay cv: <12.9%
intra-assay cv: <4.9%
standard curve range: 1.17-300 pg/mL
accuracy: 84-95%

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

UniProt accession no.

P13500

application(s)

research use

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Gene Information

human ... CCL2(6347)

Related Categories

General description

Human MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. In particilar this is a marker of inflammatory disorders. It binds to chemokine receptors CCR2 and CCR4.
This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human MCP-1 Conferma® ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Packaging

Two boxes; each to be analyzed separately.

Analysis Note

3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

recommended

Product No.
Description
Pricing

HSTCMAG-28SK

MILLIPLEX® Human High Sensitivity T Cell Panel - Immunology Multiplex Assay, Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

HCYTA-60K

MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A - Immunology Multiplex Assay

03-0187-00

SMC® Human MCP-1 High Sensitivity Immunoassay Kit

EZIL6

Human IL-6 Conferma® ELISA, quantification of human IL-6 in serum, plasma, or culture media samples

EZHIL8

Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

40-122

Belysa Immunoassay Curve Fitting Software

EZHTNFA-150K

Human TNF-α Conferma® ELISA Kit, kit sufficient for 96 wells

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Conferma® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Protocols

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Related Content

Immunoassays for Cosmetics and Personal Care Research

Learn how immunoassays are used in cosmetics and personal care research to discover potential harmful effects of cosmetics products, and assess markers of inflammation, sensitization, aging, and tissue regeneration among others.

Neuroimmunology & Neuroinflammation Immunoassays

Find immunoassays to explore neuroimmunology and neuroinflammation cytokines and neurodegenerative disease biomarkers.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service