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EZHS40

Human Amyloid β40 ELISA Kit

Name: Human Amyloid β40 ELISA Kit
Brand: MM

measures and quantifies Amyloid β40 levels in 50 μL CSF, cell culture supernatent or plasma

Synonym(s):

Amyloid-beta protein 40

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About This Item

UNSPSC Code:

12161503

eCl@ss:

32161000

NACRES:

NA.84

product name

High Sensitivity Human Amyloid β40 ELISA, This High Sensitivity Human Amyloid β40 ELISA is used to measure & quantify Amyloid β40 levels in Neuroscience research.

Quality Level

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (Overnight assay)

assay range

sensitivity: 6.0 pg/mL
(50 μl sample size)
standard curve range: 16-500 pg/mL

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cerebrospinal fluid (CSF)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... APP(351)

Related Categories

Enzyme-Linked Immunosorbent Assay (ELISA) Kits

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s High Sensitivity Human Amyloid β40 ELISA kit is used for the measurement of Amyloid β40 in cerebrospinal fluid, cell culture supernatants, primary neurons and plasma in a 96-well format.

Specificity

The Amyloid β40 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Application

Research Category
Neuroscience

Research Sub Category
Alzheimer′s Disease

This High Sensitivity Human Amyloid β40 ELISA is used to measure & quantify Amyloid β40 levels in Neuroscience research.

This assay requires 50 µl of sample (cerebrospinal fluid, cell culture supernatants, primary neurons, or plasma) and is an overnight assay.

Used to detect/quantify: Amyloid β40

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Warning

Hazard Statements

H290,H317,H412

Precautionary Statements

P234 - P261 - P272 - P273 - P280 - P302 + P352

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

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Overexpression of mutant amyloid-β protein precursor and presenilin 1 modulates enteric nervous system.

Kendra L Puig et al.

Journal of Alzheimer's disease : JAD, 44(4), 1263-1278 (2014-11-20)

Alzheimer's disease (AD) is a neurodegenerative disorder histologically characterized by amyloid-β (Aβ) protein accumulation and activation of associated microglia. Although these features are well described in the central nervous system, the process and consequences of Aβ accumulation in the enteric

Region-specific dendritic simplification induced by Aβ, mediated by tau via dysregulation of microtubule dynamics: a mechanistic distinct event from other neurodegenerative processes.

Nataliya Golovyashkina et al.

Molecular neurodegeneration, 10, 60-60 (2015-11-07)

Dendritic simplification, a key feature of the neurodegenerative triad of Alzheimer's disease (AD) in addition to spine changes and neuron loss, occurs in a region-specific manner. However, it is unknown how changes in dendritic complexity are mediated and how they

Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer's Disease.

Gunjan Manocha et al.

Current Alzheimer research, 15(12), 1123-1135 (2018-08-03)

Alzheimer's disease (AD) is associated with age-associated central nervous system degeneration and dementia. This decline in the function correlates with deposition of Aβ peptide containing plaques and associated reactive gliosis. The inflammatory phenotype of microglia, in particular, is often considered

Development of Immunoassays for the Quantitative Assessment of Amyloid-β in the Presence of Therapeutic Antibody: Application to Pre-Clinical Studies.

Anna Bogstedt et al.

Journal of Alzheimer's disease : JAD, 46(4), 1091-1101 (2015-09-25)

Utilizing decision making biomarkers in drug development requires thorough assay validation. Special considerations need to be taken into account when monitoring biomarkers using immunoassays in the presence of therapeutic antibodies. We have developed robust and sensitive assays to assess target

Chronic Presence of Oligomeric Aβ Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression.

Mariya V Hrynchak et al.

Frontiers in synaptic neuroscience, 12, 16-16 (2020-05-12)

Alzheimer's disease is regarded as a synaptopathy with a long presymptomatic phase. Soluble, oligomeric amyloid-β (Aβ) is thought to play a causative role in this disease, which eventually leads to cognitive decline. However, most animal studies have employed mice expressing

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