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ES-002

EmbryoMax® 2X Freezing Medium for ES Cells

Formulated with 20% DMSO & Fetal Bovine Serum and available in a 10 x 10 mL format.

Synonym(s):

ES Cell Freezing Medium, EmbryoMax Freezing Medium, EmbryoMax Freezing Solution

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
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Product Name

EmbryoMax® 2X Freezing Medium for ES Cells, formulated with 20% DMSO & Fetal Bovine Serum, The EmbryoMax 2X Freezing Medium for ES Cells is formulated with 20% DMSO & Fetal Bovine Serum and available in a 10 x 10 mL format.

Quality Level

200, 300, 100

form

liquid

manufacturer/tradename

Specialty Media, EmbryoMax®

technique(s)

cell culture | stem cell: suitable, cryopreservation: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

General description

This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data). Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal Bovine Serum. The reagents used in these products are certified to be virus and mycoplasma free.

The sera utilized in this medium is approved by the United States Department of Agriculture (USDA) to ensure that sera is free of diseases such as Bovine Spongiform Encephalopathy (BSE).

Packaging

10 x 10 mL

Preparation Note

Store at -80°C up to 2 years

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Articles

Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines

Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.

Related Content

Murine Embryonic Stem Cell Culture Procedures & Protocols

The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

Global Trade Item Number

SKUGTIN
ES-002-5F04053252408816
ES-002-D04053252284519
ES-002-10F04053252325960