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EQCYTMG-93KPX23

Millipore

MILLIPLEX® Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay

The Equine Inflammation / Immunology Bead-Based Multiplex Cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

horse

manufacturer/tradename

Milliplex®

assay range

accuracy: 76%
(IL-6)
accuracy: 78%
(FGF-2)
accuracy: 82%
(G-CSF)
accuracy: 85%
(IL-13)
accuracy: 87%
(TNFα)
accuracy: 88%
(RANTES/CCL5)
accuracy: 90%
(IL-2)
accuracy: 92%
(GM-CSF)
accuracy: 94%
(IL-18)
accuracy: 97%
(GRO)
sensitivity: 1.4 pg/mL
(IL-6)
sensitivity: 10.6 pg/mL
(IL-5)
sensitivity: 13.5 pg/mL
(IL-2)
sensitivity: 15.4 pg/mL
(IL-17A)
sensitivity: 16.3 pg/mL
(FGF-2)
sensitivity: 16.7 pg/mL
(IP-10)
sensitivity: 165 pg/mL
(IFNγ)
sensitivity: 25.3 pg/mL
(IL-18)
sensitivity: 29.3 pg/mL
(IL-1β)
sensitivity: 3.9 pg/mL
(RANTES/CCL5)
sensitivity: 3.94 pg/mL
(GM-CSF)
sensitivity: 340 pg/mL
(G-CSF)
sensitivity: 355 pg/mL
(IL-4)
sensitivity: 39-130 pg/mL
(IL-13)
sensitivity: 39.1 pg/mL
(IL-1α)
sensitivity: 399 ng/mL
(MCP-1)
sensitivity: 457 pg/mL
(Fractalkine/CS3CL1)
sensitivity: 49.2 pg/mL
(IL-12 (p70))
sensitivity: 5.2 pg/mL
(TNFα)
sensitivity: 62.5 pg/mL
(IL-10)
sensitivity: 7.8 pg/mL
(GRO)
sensitivity: 74.3 pg/mL
(IL-8/CXCL8)
sensitivity: 8.3 pg/mL
(Eotaxin/CCL11)
standard curve range: 15-15,000 pg/mL
(IL-17A)
standard curve range: 15-15,000 pg/mL
(IL-2)
standard curve range: 15-15,000 pg/mL
(IL-5)
standard curve range: 15-15,000 pg/mL
(IL-6)
standard curve range: 15-15,000 pg/mL
(IP-10)
standard curve range: 24-25,000 pg/mL
(FGF-2)
standard curve range: 29-30,000 pg/mL
(IL-1β)
standard curve range: 293-300,000 pg/mL
(IL-4)
standard curve range: 34-35,000 pg/mL
(IL-18)
standard curve range: 342-350,000 ng/mL
(MCP-1)
standard curve range: 39-40,000 pg/mL
(IL-12 (p70))
standard curve range: 39-40,000 pg/mL
(IL-13)
standard curve range: 4-4,000 pg/mL
(TNFα)
standard curve range: 488-500,000 pg/mL
(IFNγ)
standard curve range: 49-50,000 pg/mL
(IL-10)
standard curve range: 5-5,000 pg/mL
(GM-CSF)
standard curve range: 5-5,000 pg/mL
(RANTES/CCL5)
standard curve range: 586-600,000 pg/mL
(Fractalkine/CS3CL1)
standard curve range: 59-60,000 pg/mL
(IL-1α)
standard curve range: 59-60,000 pg/mL
(IL-8/CXCL8)
standard curve range: 6-6,000 pg/mL
(GRO)
standard curve range: 7-7,000 pg/mL
(Eotaxin/CCL11)
standard curve range: 78-80,000 pg/mL
(G-CSF)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is as true for horses as it is for humans. Both experience immunological diseases, such as infectious diseases, osteoarthritis, and respiratory diseases, as well as neurologic, metabolic, and cardiovascular diseases and cancer. Until now the ability to measure levels of cytokines and chemokine in horses had been limited primarily to ELISAs. Researchers can now measure multiple inflammation biomarkers simultaneously using the MILLIPLEX® Equine Cytokine/Chemokine Panel, based on the Luminex® xMAP® technology.

MILLIPLEX® Equine Cytokine/Chemokine Premix Panel is a 23-plex kit used for the simultaneous quantification of all the following analytes in equine serum, plasma, or culture supernatant samples: Eotaxin/CCL11, FGF-2, Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10 (CXCL10), MCP-1, RANTES (CCL5), TNF-α
  • Recommended Sample Type: Equine serum, plasma, tissue culture supernatant or tissue/cell extracts
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require dilution with an appropriate medium
  • Assay Run Time: Overnight or One Day A primary incubation of 2 hours at room temperature (RT) (20-25°C), or for greater sensitivity, overnight (16-18 hours) at 2-8°C; followed by a 1 hour secondary incubation at RT
  • Research Category: Inflammation & Immunology

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Regulatory Information

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Hannah C Valigura et al.
Journal of animal science, 99(8) (2021-06-29)
Mitigation of exercise-induced stress is of key interest in determining ways to optimize performance horse health. To test the hypothesis that dietary supplementation of a Saccharomyces cerevisiae fermentation product would decrease markers of exercise-induced stress and inflammation in young horses

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