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DR1094

Sigma-Aldrich

Anti-PAX7 Mouse mAb (1E12)

liquid, clone 1E12, Calbiochem®

Synonym(s):

Anti-Paired Box 7

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1E12, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PAX7(5081)

General description

Purified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
Recognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.
This Anti-PAX7 Mouse mAb (1E12) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PAX7.

Immunogen

A recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein

Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.5 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
NIH3T3 cells, Human stomach tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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WGK

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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