CCYTOMAG-90K

MILLIPLEX^® Canine Cytokine/Chemokine Panel

Name: MILLIPLEX<SUP>®</SUP> Canine Cytokine/Chemokine Panel
Brand: MM

Configurable Canine Cytokine/Chemokine 13-Plex Panel

Synonym(s):

canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit

About This Item

UNSPSC Code:

12161503

NACRES:

NA.47

eCl@ss:

32161000

Key Documents

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Product Name

MILLIPLEX^® Canine Cytokine/Chemokine Panel, Configurable Canine Cytokine/Chemokine 13-Plex Panel

species reactivity

canine

manufacturer/tradename

Milliplex^®

Quality Level

200

packaging

1 ea of

assay range

accuracy: 91-109%
sensitivity: 13.6 pg/mL
(IFN-γ)
sensitivity: 21.0 pg/mL
(MCP-1)
sensitivity: 21.7 pg/mL
(IL-8)
sensitivity: 3.2 pg/mL
(IP-10)
sensitivity: 3.5 pg/mL
(IL-2)
sensitivity: 3.7 pg/mL
(IL-6)
sensitivity: 5.3 pg/mL
(KC-like)
sensitivity: 5.8 pg/mL
(IL-18)
sensitivity: 6.1 pg/mL
(TNFα)
sensitivity: 7.5 pg/mL
(IL-7)
sensitivity: 8.5 pg/mL
(IL-10)
sensitivity: 9.0 pg/mL
(IL-15)
sensitivity: 9.2 pg/mL
(GM-CSF)
standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

input

serum plasma (Cell culture, cell culture supernatants)

detection method

fluorometric (Luminex^® xMAP^® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Immunoassay Platform Solutions

MILLIPLEX^® Multiplex Assays for Luminex^® Instruments

Protein Biology

Biochem/physiol Actions

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

There was no or negligible cross-reactivity among different analytes within the panel.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

General description

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX^® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Other Notes

Replaces: CCYTO-90K

Packaging

Everything you need in a single kit.

Preparation Note

Recommended storage for kit components is 2 - 8°C

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Application

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 13 analytes in canine serum, plasma, and cell culture supernatants.Analytes included: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, TNF-αAssay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

Comprehensive 13-plex panel: Simultaneously quantifies essential inflammatory markers across multiple canine sample types including serum, plasma, cell culture supernatants, and tissue lysates.
High-throughput efficiency with sample conservation: 96-well format processes up to 38 samples in duplicate while requiring only 25 μL per test, maximizing productivity while preserving valuable biological specimens for additional analyses.
Advanced canine health and disease research: Investigate immune responses in breed-specific disorders, age-related conditions, vaccine efficacy studies, and nutritional immunology research to improve veterinary care and canine welfare outcomes.
Flexible protocol options: Choose between overnight 4°C incubation for convenience or 2-hour room temperature protocol for same-day results, accommodating diverse laboratory workflows and experimental timelines.

pictograms

GHS08,GHS05,GHS07,GHS09

signalword

Danger

hcodes

H302 + H312 + H332,H317,H318,H373,H411

pcodes

P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

新产品

This item has

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Association of Canine Osteosarcoma and Monocyte Phenotype and Chemotactic Function.

J L Tuohy et al.

Journal of veterinary internal medicine, 30(4), 1167-1178 (2016-06-25)

Monocytes/macrophages are likely key cells in immune modulation in dogs with osteosarcoma (OSA). Increased peripheral monocyte counts are negatively correlated with shorter disease-free intervals in dogs with OSA. Understanding the monocyte/macrophage's modulatory role in dogs with OSA can direct further

Effect of immunosuppressive drugs on cytokine production in canine whole blood stimulated with lipopolysaccharide or a combination of ionomycin and phorbol 12-myristate 13-acetate.

Julien R S Dandrieux et al.

Veterinary medicine and science, 5(2), 199-205 (2019-01-22)

A pharmacodynamic assay has been previously developed to monitor ciclosporin treatment in dogs by assessing inhibition of cytokine transcription after whole blood stimulation with 12-myristate 13-1 acetate and ionomycin (PMA/I). In this study, whole blood stimulation with either PMA/I or

Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as Prognostic Markers in Mammary Tumors.

Gustavo Rodrigues Martins et al.

Mediators of inflammation, 2016, 9512743-9512743 (2016-03-19)

Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between

Antimicrobial and anti-inflammatory thermo-reversible hydrogel for periodontal delivery.

Bing Wang et al.

Acta biomaterialia, 116, 259-267 (2020-09-17)

In periodontal treatment, topical adjunctive therapy with antimicrobials or anti-inflammatory agents is frequently applied. However, currently available drug carrier biomaterials often exhibit poor perfusion into small crevices, such as the deep and irregular periodontal pockets, due to relatively high viscosity.

Intra-articular Administration of Allogeneic Adipose Derived MSCs Reduces Pain and Lameness in Dogs With Hip Osteoarthritis: A Double Blinded, Randomized, Placebo Controlled Pilot Study.

Chad B Maki et al.

Frontiers in veterinary science, 7, 570-570 (2020-10-29)

This study was conducted to investigate the therapeutic effect of allogeneic adipose-derived MSCs on dogs with hip osteoarthritis (OA). Twenty dogs with bilateral osteoarthritis of the coxofemoral (hip) joint, diagnosed by a veterinarian through physical examination and radiographs were randomly

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MILLIPLEX® Canine Cytokine/Chemokine Panel