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CCYTMAG90KPX13BK

Millipore

MILLIPLEX® Canine Cytokine/Chemokine Magnetic Bead Panel - Premixed 13 Plex Space Saver (Bulk) Packaging

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in rat serum, plasma and cell culture samples.

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Synonym(s):
canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

canine

manufacturer/tradename

Milliplex®

assay range

accuracy: 101%
(IL-8)
accuracy: 102%
(IL-7)
accuracy: 109%
(IL-15)
accuracy: 95%
(GM-CSF)
accuracy: 96%
(IL-18)
accuracy: 98%
(IL-6)
sensitivity: 13.6 pg/mL
(IFN-γ)
sensitivity: 21.0 pg/mL
(MCP-1)
sensitivity: 21.7 pg/mL
(IL-8)
sensitivity: 3.2 pg/mL
(IP-10)
sensitivity: 3.5 pg/mL
(IL-2)
sensitivity: 3.7 pg/mL
(IL-6)
sensitivity: 5.3 pg/mL
(KC-like)
sensitivity: 5.8 pg/mL
(IL-18)
sensitivity: 6.1 pg/mL
(TNFα)
sensitivity: 7.5 pg/mL
(IL-7)
sensitivity: 8.5 pg/mL
(IL-10)
sensitivity: 9.0 pg/mL
(IL-15)
sensitivity: 9.2 pg/mL
(GM-CSF)
standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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General description

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
UPDATE >>
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-α.
  • Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
  • Recommended Sample Dilution: 25 μL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25° C)
  • Research Category: Inflammation & Immunology

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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