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Sigma-Aldrich

Human Collagen Type III

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

Synonym(s):

Collagen III from human placenta, alpha 1 Type III Collagen

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Human Collagen Type III,

biological source

human

Quality Level

100

Assay

90% (Human collagen type III, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<1% Human collagen types II, IV-VI, and non-collagen proteins
<10% Human collagen type I

input

sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)

solubility

water: soluble at 20 °C

NCBI accession no.

NM_000090.3

UniProt accession no.

P02461

Binding Specificity

Peptide Source: Fibrinogen

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COL3A1(1281)

Related Categories

General description

The COL3A1 gene mapped to human chromosome 2q32.2, encodes the alpha 1 chain of type III collagen. Type III collagen is an extracellular matrix protein containing a long triple-helical domain made of three alpha 1 chains. Collagen III is a major structural component in hollow organs such as large blood vessels, the uterus and the bowel. Human type III collagen is purified by serial salt precipitations, alcohol precipitation, and DEAE chromatography of an acetic acid extraction of human placenta, following mild pepsin digestion. Product Source: Human placenta, negative for HbsAG, HCV and HIV 1 & 2 antibodies.

Application

Human Collagen Type III has been used:
  • as an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slides for probing and detection experiments
  • to coat microfluidic migration devices and to study the impact of extracellular matrix (ECM) on cell migration
  • as a positive control in Gpr56 PRESTO-Tango assay and GTP-Rho pull-down assay, to evaluate its effects on cellular signaling and interactions

Biochem/physiol Actions

Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. The type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen tissue strengthening function

Physical form

Liquid in 0.5 M acetic acid, pH 2.5.

Storage and Stability

Maintain at -20ºC in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Other Notes

Composition: [a1(III)]3, native triple helix. Ability to form native helical structure verified by ORD measurement, competence in microfibril formation and reactivity with anti-collagen type-specific monoclonal antibodies

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品-Merck

Certificates of Analysis (COA)

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Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases
Kuivaniemi H, et al.
A Manual for Genetic Engineering: Advanced Bacterial Genetics, 707, 151-171 (2019)
Phenotype of COL3A1/COL5A2 deletion patients
Kempers MJ, et al.
European Journal of Medical Genetics, 65(10), 104593-104593 (2022)
Microfluidic single-cell migration chip reveals insights into the impact of extracellular matrices on cell movement
Zhou M, et al.
Lab on a chip, 23(21), 4619-4635 (2023)
Sarah D Ackerman et al.
The Journal of experimental medicine, 215(3), 941-961 (2018-01-26)
Myelin is a multilamellar sheath generated by specialized glia called Schwann cells (SCs) in the peripheral nervous system (PNS), which serves to protect and insulate axons for rapid neuronal signaling. In zebrafish and rodent models, we identify GPR56/ADGRG1 as a
Guido Carpino et al.
Clinical proteomics, 16, 37-37 (2019-11-07)
Intrahepatic cholangiocarcinoma (iCCA) is a malignancy that arises from the intrahepatic biliary tree, showing high mortality rates due to its late clinical presentation and limited treatment options. iCCA is characterized by a dense, reactive desmoplastic stroma marked by a dramatic
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This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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