CBL251
Anti-Epithelial Specific Antigen Antibody, clone VU-1D9
clone VU-1D9, from mouse
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Synonym(s):
Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastro
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
VU-1D9, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Epithelial specific antigen, also known as epithelial cell adhesion molecule (Ep-CAM) and epithelial glycoprotein (EGP), may act as an interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Epithelial specific antigen plays a role in embryonic stem cells proliferation and differentiation, and up-regulates the expression of FABP5, MYC, and cyclins A and E. Epithelial specific antigen is highly and selectively expressed by undifferentiated embryonic stem cells (ESCs). Protein levels rapidly diminish as soon as ESCs differentiate. Also expressed in most epithelial cell membranes and on the surface of adenocarcinoma.
Immunogen
NCI-H69 whole cells
Application
Anti-Epithelial Specific Antigen Antibody, clone VU-1D9 is a highly specific mouse monoclonal antibody, that targets Epithelial Cell Marker & has been tested in western blotting, ICC & IHC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Epithelial Specific Antigen in A431 cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Quality
Evaluated by Western Blotting in MCF7 cell lyate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.
Target description
~40 kDa observed. The calculated molecular weight of this protein is 35 kDa; however, this protein will run higher due to glycosylation.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
MCF7 cell lyasate
MCF7 cell lyasate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shaohua Wang et al.
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