CBL218
Anti-Keratin K3/K76 Antibody, clone AE5
clone AE5, Chemicon®, from mouse
Synonym(s):
Anti-HUMCYT2A, Anti-KRT2P
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Keratin K3/K76 Antibody, clone AE5, clone AE5, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AE5, monoclonal
species reactivity
human, mouse, bovine, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KRT3(3850)
Biochem/physiol Actions
The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
General description
64 kDa
Immunogen
Rabbit corneal epithelial keratin
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.
Preparation Note
For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
Immunohistochemistry on frozen sections: 1:50.
Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).
Western blotting: 1:500.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).
Western blotting: 1:500.
Optimal working dilutions must be determined by the end user.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Fiona J Li et al.
Experimental eye research, 147, 1-11 (2016-04-18)
The New Zealand White rabbit has been widely used as a model of limbal stem cell deficiency (LSCD). Current techniques for experimental induction of LSCD utilize caustic chemicals, or organic solvents applied in conjunction with a surgical limbectomy. While generally
Ashkon G Seyed-Safi et al.
Experimental eye research, 197, 108063-108063 (2020-05-18)
Limbal epithelial stem cells are required for the maintenance and repair of the corneal epithelial surface. The difficulty in obtaining human corneal tissue for research purposes means that animal models for studying the corneal and limbal epithelium are extremely useful.
Xiaojun Lian et al.
PloS one, 8(3), e60016-e60016 (2013-03-26)
Human pluripotent stem cells (hPSCs) provide unprecedented opportunities to study the earliest stages of human development in vitro and have the potential to provide unlimited new sources of cells for regenerative medicine. Although previous studies have reported cytokeratin 14+/p63+ keratinocyte
Jeremy John Mathan et al.
Stem cell research & therapy, 7(1), 81-81 (2016-06-03)
The limbus forms the outer rim of the cornea at the corneoscleral junction and harbours a population of stem cells for corneal maintenance. Injuries to the limbus, through disease or accidents such as chemical injuries or burns, may lead to
Cristina Romo-Valera et al.
Experimental eye research, 211, 108720-108720 (2021-08-15)
The transplantation of expansions of limbal epithelial stem cells (LESC) remains one of the most efficient therapies for the treatment of limbal stem cell deficiency (LSCD) to date. However, the available donor corneas are scarce, and the corneas conserved for
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