CBL180-I
Anti-CD62E [ELAM-1] Antibody, clone 1.2B6
clone 1.2B6, from mouse
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E-selectin, CD62 antigen-like family member E, CD62E, ELAM-1, Endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, P-selectin, CD62 antigen-like family member P, CD62P, GMP-140, Granu
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1.2B6, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
activity assay: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SELE(6401)
Related Categories
General description
Selectins (CD62s) are single-chain transmembrane glycoproteins that function as cell adhesion molecules (CAMs) by interacting with a range of oligosaccharide structures including sialyl-Lewis X (sLeX) via their C-type lectin domain. All three known selectins (L-, E-, and P-selectin or CD62L CD62E, CD62P, respectively) share a similar overall domain structure that includes an N-terminal C-type lectin domain (calcium-dependent lectin domain), an EGF-like domain, a variable number of short consensus repeat (SCR) or Sushi domains (2, 6, and 9 for CD62L CD62E, CD62P, respectively), a transmembrane domain, and a cytoplasmic tail. The extracellular domains show a high homology among the three selectins, the transmembrane and cytoplasmic domains however are not conserved among the three. E-selectin (CD62E, ELAM-1) and P-selectin (CD62P, GMP-140, LECAM3, PADGEM) are mainly expressed on endothelial cells at inflammatory sites, whereas L-selectin (CD62L) is exclusively expressed on leukocytes.
Specificity
This antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. This antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab′)2 fragments. Although previously thought to recognize only human CD62E, later data (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560) showed that this antibody also recognizes human CD62P by binding to a common epitope on these members of the selectin family.
Application
Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in Immunohistochemistry, ELISA, Flow Cytometry, Western Blotting, Activity Assay.
ELISA Analysis: A representative lot detected an induction of E-selectin expression on HUVECs by T-cells isolated from human PBMCs (Yarwood, H., et al. (2000). J. Leukoc. Biol. 68(2):233-242).
ELISA Analysis: A representative lot detected a time-dependent induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs, but not A549 cells (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Flow Cytometry Analysis: A representative lot specifically reacted with exogenously expressed human E- and P-, but not L-, selectin on the surface of transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Flow Cytometry Analysis: A representative lot detected an induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Western Blotting Analysis: A representative lot specifically reacted with E- and P-, but not L-, selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked the adhesion of HL-60 cells to E- or P-selectin transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked sialyl-Lewis X (sLeX) binding by E- and P-selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Immunohistochemistry Analysis: A representative lot detected ELAM-1 immunoreactivity in acetone-fixed frozen human skin tissue sections (Groves, R.W., et al. (1991). Br. J. Dermatol. 124(2):117-123).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E-selectin and associated proteins from lysates of TNF-stimulated HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
ELISA Analysis: A representative lot detected a time-dependent induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs, but not A549 cells (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Flow Cytometry Analysis: A representative lot specifically reacted with exogenously expressed human E- and P-, but not L-, selectin on the surface of transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Flow Cytometry Analysis: A representative lot detected an induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Western Blotting Analysis: A representative lot specifically reacted with E- and P-, but not L-, selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked the adhesion of HL-60 cells to E- or P-selectin transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked sialyl-Lewis X (sLeX) binding by E- and P-selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Immunohistochemistry Analysis: A representative lot detected ELAM-1 immunoreactivity in acetone-fixed frozen human skin tissue sections (Groves, R.W., et al. (1991). Br. J. Dermatol. 124(2):117-123).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E-selectin and associated proteins from lysates of TNF-stimulated HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Quality
Evaluated by Immunohistochemistry in human tonsil tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CD62E [ELAM-1] in human tonsil tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CD62E [ELAM-1] in human tonsil tissue.
Target description
66 kDa calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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