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usage
sufficient for 96 tests
Quality Level
packaging
pkg of 1 96-well plate(s)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
input
sample type monocyte(s)
detection method
fluorometric
shipped in
wet ice
Related Categories
General description
The InnoCyte Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.
Components
96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.
Warning
Toxicity: Irritant (B)
Principle
The Calbiochem InnoCyte 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.
Preparation Note
Cell Labeling Mixture: Warm the D-PBS and Calcein-AM Solution to room temperature. Add 35 µl Calcein-AM Solution to 10 ml D-PBS. Mix well. Note: Prepare just before use (see step 7 of the Detailed Protocol below).
Storage and Stability
Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
Other Notes
Cravens, P.D. 2007. Scan. J. Immunol.65, 514.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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