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CB1020

Sigma-Aldrich

Anti-MFN2 Mouse mAb (4H8)

liquid, clone 4H8, Calbiochem®

Synonym(s):

Anti-Mitofusin 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

4H8, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Mfn2(64476)

General description

Purified mouse monoclonal antibody. Recognizes the ~75 kDa MFN2 protein.
Recognizes the ~75 kDa MFN2 protein in HeLa and PC-12 cells and human heart tissue.
This Anti-MFN2 Mouse mAb (4H8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of MFN2.

Immunogen

A recombinant polypeptide corresponding to amino acids 661-758 of human MFN2, expressed as a GST fusion protein
NA

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (5 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells, PC-12 cells, Human heart tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Moran, M., et al. 2010. Biochim Biophys. Acta.1802 443.
Holloway, G,P., et al. 2008. Am. J. Physiol. Endocrinol. Metab.294 E1060.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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