APT129
Caspase 8 Colorimetric Activity Assay Kit 25, IETD
Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.
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Synonym(s):
FLICE
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Recommended Products
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
detection method
colorimetric
shipped in
dry ice
General description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Components
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution
Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
Storage and Stability
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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