AP307P
Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate
1 mg/mL, Chemicon®
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
Specificity
Specific for rabbit IgG, heavy and light chain. The cross-reactivities of anti-rabbit IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
Application
Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate has been used in:
- immunohistochemistry (IHC)
- western blotting
- immunoprecipitation
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Quality
Western Blot, chromogenic:
1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).
Western Blot, chemiluminescent:
1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).
1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).
Western Blot, chemiluminescent:
1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).
Physical form
ImmunoAffinity Purified
Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH 7.1 with 15mg/mL BSA and 0.1% Kathon.
Storage and Stability
The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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