AP182P
Donkey Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed
Chemicon®, from donkey
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
donkey
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
Rabbit IgG
Application
Donkey anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in ELISA & WB.
EIA and Western blots: 1:5,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000.
Western blotting with ECL substrates: 1:10,000-1:200,000.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:500-1:5,000.
Western blotting with ECL substrates: 1:10,000-1:200,000.
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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