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Sigma-Aldrich

Rabbit Anti-Sheep IgG Antibody, biotin-SP conjugate

Chemicon®, from rabbit

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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

rabbit

Quality Level

100

conjugate

biotin conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

sheep

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

Specificity

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum protein, but the antibody may cross-react with immunoglobulins from other species.

Immunogen

Purified Sheep IgG.

Application

DILUTION:

1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin

1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin

1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections

1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

Optimal working dilution must be determined by the end user.
Rabbit anti-Sheep IgG Antibody, biotin-SP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

ImmunoAffinity Purified
Lyophilized. Buffer = 0.01M Phosphate buffer, 0.25 M NaCl with 15 mg/mL BSA and 0.05% sodium azide.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months from date of receipt. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Expiration date: one year from date of reconstitution.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Osvaldo Giorgi et al.
Journal of neuroscience research, 93(5), 714-721 (2014-12-17)
Roman high (RHA)- and low (RLA)-avoidance rats are selectively bred for rapid vs. poor acquisition of active avoidance, respectively, and differ markedly in emotional reactivity, coping style, and behavioral and neurochemical responses to morphine and psychostimulants. Accordingly, acute cocaine induces

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