AP136P
Goat Anti-Rat IgG Antibody, HRP conjugate
Chemicon®, from goat
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Goat anti-rat IgG affinity purified antibody, HRP conjugated.
Specificity
Specific for rat IgG, heavy and light chain.
Application
Detect Rat IgG using this Goat anti-Rat IgG Antibody, HRP conjugate validated for use in ELISA, IH & WB.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Quality
Routinely evaluated on Enzyme-linked Immunosorbent Assay.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used..
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used..
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitute with 2 mL of sterile distilled water.
Reconstitute with 2 mL of sterile distilled water.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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