AP1063
PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Pyruvate Dehydogenase pSer²³² Rabbit pAb
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer232)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α
Application
Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HEK293 cells
HEK293 cells
Other Notes
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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