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Merck
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ACK5030NT

Amicon® Pro Affinity Concentration Kit Ni-NTA with 30kDa Amicon® Ultra-0.5 Device

Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.

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About This Item

eCl@ss:
36100101
UNSPSC Code:
41104916
Material:
Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)
Pore size:
30 kDa NMWCO
Working volume:
10 mL
Sterility:
non-sterile

Key Documents

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material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Pro

parameter

10 mL sample volume

technique(s)

buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration), protein purification: suitable (enrichment)

L

12 cm (4.7 in.)

diam.

2.97 cm

filtration area

1 cm2

working volume

10 mL

pore size

30 kDa NMWCO

shipped in

wet ice

Related Categories

General description

Filter Code: PLCGC
Filter Type: Ultrafiltration
This kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 30 kDa, 3 ml HisBind Ni-NTA resin (Binding capacity: 5 to 10mg His tagged protein/ml agarose), 25ml Bind Buffer (4X), 25 ml Wash buffer (4X), 5ml elution buffer (4X)

Application

Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.

Features and Benefits

Minimum Final Concentrate Volume: 15 µL

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Skin Corr. 1C

Storage Class

3 - Flammable liquids

flash_point_f

95.9 °F

flash_point_c

35.5 °C

Regulatory Information

新产品
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Certificates of Analysis (COA)

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Amicon®Pro Purification System

Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

Amicon® Pro Affinity Concentration Kit - Ni-NTA
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