ABT69
Anti-CCDC53 Antibody
from rabbit
Synonym(s):
coiled-coil domain containing 53, Coiled-coil domain-containing protein 53, WASH complex subunit CCDC53
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CCDC53(51019)
General description
Coiled-coil domain-containing protein 53 (CCDC53) is one of several components comprising the WASH complex, a complex localized to endosomal surfaces that functions to upregulate actin polymerization through the recruiting and activation of Arp2/3 complex. WASH is a critical component in the fission of tubules (transport intermediates involved in the distribution of endosomes). The WASH complex is composed of CCDC53, F-actin-capping protein subunits alpha and beta, WASH, FAM21, KIAA1033, and KIAA196.
Immunogen
GST-tagged recombinant protein corresponding to human CCDC53.
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
This Anti-CCDC53 Antibody is validated for use in WB for the detection of CCDC53.
Western Blot Analysis: 2.5 µg/mL from a representative lot detected CCDC53 in 10 µg of mouse brain and PC12 cell lysate.
Quality
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 2.5 µg/mL of this antibody detected CCDC53 in 10 µg of HepG2 cell lysate.
Western Blot Analysis: 2.5 µg/mL of this antibody detected CCDC53 in 10 µg of HepG2 cell lysate.
Target description
~28 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HepG2 cell lysate
HepG2 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Vojtěch Dostál et al.
Traffic (Copenhagen, Denmark), 24(5), 216-230 (2023-03-31)
The pentameric WASH complex facilitates endosomal protein sorting by activating Arp2/3, which in turn leads to the formation of F-actin patches specifically on the endosomal surface. It is generally accepted that WASH complex attaches to the endosomal membrane via the
Benjamin J Tyrrell et al.
Pigment cell & melanoma research, 29(5), 559-571 (2016-07-09)
The five-subunit WASH complex generates actin networks that participate in endocytic trafficking, migration and invasion in various cell types. Loss of one of the two subunits WASH or strumpellin in mice is lethal, but little is known about their role
Ewan MacDonald et al.
The Journal of cell biology, 217(7), 2549-2564 (2018-06-13)
Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In
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