ABT58
Anti-phospho PKA-RII (Ser96) Antibody
from rabbit, purified by affinity chromatography
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PRKAR2A, protein kinase, cAMP-dependent, regulatory, type II, alpha
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer96)
Gene Information
human ... PRKAR2A(5576)
General description
cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.
Specificity
The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.
Immunogen
Epitope: Phosphorylated Ser96 of PKA-RIIalpha
KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.
Application
Research Category
Cell Structure
Signaling
Cell Structure
Signaling
Research Sub Category
Cytoskeletal Signaling
Kinases & Phosphatases
Cytoskeletal Signaling
Kinases & Phosphatases
This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).
Quality
Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.
Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.
Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.
Target description
~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.
Linkage
Replaces: 06-704
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Forskolin untreated and treated PC12 cell lysates
Forskolin untreated and treated PC12 cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Translational psychiatry, 6(9), e881-e881 (2016-09-07)
Intracellular cAMP and serotonin are important modulators of anxiety and depression. Fluoxetine, a selective serotonin reuptake inhibitor (SSRI) also known as Prozac, is widely used against depression, potentially by activating cAMP response element-binding protein (CREB) and increasing brain-derived neurotrophic factor
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