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ABT48

Sigma-Aldrich

Anti-Vesicle protein sorting 35 (VPS35) Antibody

from rabbit

Synonym(s):

vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35, Maternal-embryonic 3, vacuolar protein sorting 35 (yeast homolog), vacuolar protein sorting 35 (yeast), Vesicle protein sorting 35

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

technique(s)

western blot: suitable

NCBI accession no.

NP_060676

UniProt accession no.

Q96QK1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VPS35(55737)

General description

Vacuolar protein sorting-associated protein 35 (VPS35) is critical component found within the retromer complex. The retromer complex is comprised of several proteins and has been implicated in roles involving the Wnt signaling pathway, cell migration and adhesion, and polymeric immunoglobulin A transcytosis. VPS35 plays an essential role in the selection and trafficking of lysosomal enzyme receptors from endosomes to the Golgi. It is also a required component for the secretion of active Wnt. Expression of VPS35 is pervasive throughout all tissue. Highest levels of expression have been observed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, spleen, testis, and thymus.

Immunogen

GST-tagged recombinant protein corresponding to human VPS35.

Application

Anti-Vesicle protein sorting 35 (VPS35) Antibody is an antibody against Vesicle protein sorting 35 (VPS35) for use in WB.
RNAi: A previous lot of antibody was used in siRNA knockdown experiments by an independent laboratory (Courtesy of Gomez, TS, Mayo Clinic, 2010).
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected VPS35 in 10 µg of A431 cell lysate.

Target description

~ 80 kDa observed MW. Expected MW is 92 kDa

Physical form

Format: Purified
Protein G
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elpida Tsika et al.
Human molecular genetics, 23(17), 4621-4638 (2014-04-18)
Mutations in the vacuolar protein sorting 35 homolog (VPS35) gene at the PARK17 locus, encoding a key component of the retromer complex, were recently identified as a new cause of late-onset, autosomal dominant Parkinson's disease (PD). Here we explore the
Mian Cao et al.
Neuron, 93(4), 882-896 (2017-02-24)
Synaptojanin 1 (SJ1) is a major presynaptic phosphatase that couples synaptic vesicle endocytosis to the dephosphorylation of PI(4,5)P2, a reaction needed for the shedding of endocytic factors from their membranes. While the role of SJ1's 5-phosphatase module in this process

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