ABT45
Anti-Claudin-5 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Claudin-5 Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, horse, human
species reactivity (predicted by homology)
equine (based on 100% sequence homology), ox (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CLDN5(7122)
Related Categories
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Immunogen
KLH-conjugated linear peptide corresponding to human Claudin-5 at the cytoplasmic domain.
Epitope: Cytoplasmic Domain
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Analysis Note
Control
Human lung tissue lysate
Human lung tissue lysate
Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate.
Application
Anti-Claudin-5 Antibody detects level of Claudin-5 & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:800 dilution from a representative lot detected Clausin-5 in prostate tissue.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Biochem/physiol Actions
This antibody recognizes Claudin-5 at the cytoplasmic domain.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Claudin-5, a transmembrane protein deleted in velo-cardio-facial syndrome (TMVCF), seems to be involved in endothelial tight junctions. Claudin-5 has been exclusively found in the cell-cell borders of endothelial cells. Lower claudin-5 levels in sinusoidal endothelial cells could lead to their dysfunction during liver injuries. Levels of claudin-5 in tumor vessels could be a potential marker for hepatocellular carcinoma prognosis. The loss of claudin-5 function has been linked to carcinogenesis.
~17 kDa observed.
Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288).
Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288).
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
This item has
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Qian Wu et al.
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Hemorrhagic transformation (HT) has been reported to be associated with a poor prognosis after acute ischemic stroke. Blood-brain barrier (BBB) damage is considered as the major pathophysiologic mechanism of HT. Our aim was to investigate the role of acute iron
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Hongyan Zhu et al.
Neurochemical research, 43(10), 1897-1904 (2018-08-09)
Blood-brain barrier (BBB) destruction is associated with a variety of neurological diseases. Brain microvascular endothelial cells (BMECs) are the key constituent of BBB. Both matrix metalloproteinases-2/9 (MMP-2/9) and toll-like receptor-2 (TLR2) are coexpressed in BMECs and have been shown to
Karsten Bartels et al.
Heart, lung & circulation, 23(10), 981-984 (2014-06-17)
Neurologic injury is common after cardiac surgery and disruption of the blood brain barrier (BBB) has been proposed as a contributing factor. We sought to study BBB characteristics in a rodent model of cardiopulmonary bypass (CPB) and deep hypothermic circulatory
Disrupted tight junctions in the small intestine of cystic fibrosis mice.
De Lisle, RC
Cell and Tissue Research null
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