ABT331
Anti-Nephrin Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
Nephrin, NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human (immunogen homology)
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NPHS1(4868)
General description
Nephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
Immunogen
KLH-conjugated linear peptide corresponding to human Nephrin.
Application
Detect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.
Immunofluorescence Analysis: 10 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.
Immunofluorescence Analysis: 10 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.
Quality
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Nephrin in 15 µg of mouse kidney tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Nephrin in 15 µg of mouse kidney tissue lysate.
Target description
~100 kDa observed. Uncharacterized bands may appear in some lysate(s). Despite its calculated molecular weight of 135 kDa, Nephrin often migrates at a lower than expected size in SDS-PAGE.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Feng Xu et al.
Annals of translational medicine, 8(18), 1154-1154 (2020-11-27)
Diabetic nephropathy (DN) is one of the common chronic microvascular complications of diabetes, and podocyte injury and dysfunction are strictly related to the pathogenesis of DN. Studies have shown that ligustilide (LIG) has anti-inflammatory, antioxidant, and anti-apoptotic activities. This study
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