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Key Documents

ABT187

Sigma-Aldrich

Anti-TRPM8 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, LTrpC-6, LTrpC6, Transient receptor potential p8, Trp-p8, TRPM8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

baboon (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_076985

UniProt accession no.

Q7Z2W7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TRPM8(79054)

General description

Transient receptor potential cation channel subfamily M member 8 (TRPM8) is also known as Long transient receptor potential channel 6 (LTrpC-6), and Transient receptor potential p8 (Trp-p8). TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness. TRPM8 is activated by icilin, eucalyptol, menthol, cold and changes to intracellular pH. TRPM8 is expressed in prostate as well as tumors of the prostate, colon, lung, breast and skin. TRPM8 expression in most prostate tumors and the presence of an immunogenic epitope suggest that it may be suitable for the design of peptide vaccination strategies for prostate cancers.

Specificity

Other homologies: Bovine (88% sequence homology), Canine (71% sequence homology), and Mouse and Rat (53% sequence homology).
This antibody recognizes the cytoplasmic domain of TRPM8. This antibody will recognize isoform 1.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TRPM8.

Application

Detect TRPM8 using this rabbit polyclonal antibody, Anti-TRPM8 Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRPM8 in human prostate cancer and breast cancer cells.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected TRPM8 in 10 µg of LNCaP cell lysate.

Target description

~115 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
LNCaP cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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