All Photos(1)
Synonym(s):
NRAM1, NRAMP 2, NRAMP-2, Divalent metal transporter 1, Divalent cation transporter 1, DMT-1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC11A2(4891)
General description
Divalent metal ion transporter 1 (DMT1) is a proton-coupled Fe2+ transporter that is essential for iron uptake in enterocytes and for transferrin-associated endosomal iron transport in many other cell types. DMT1 dysfunction is associated with several diseases such as iron overload disorders and neurodegenerative diseases. DMT1 can transport manganese, cobalt, cadmium, nickel, and vanadium. DMT1 is also involved in iron transport from acidified endosomes into the cytoplasm in erythroid precursor cells as well as liver internal metabolism of iron. Interestingly too, in mammalian cells, mitochondria receive most incoming iron, and the outer mitochondrial membrane appears to be a major location for DMT1, which appears to be the major importer of iron into the mitochondria.
Immunogen
Recombinant protein corresponding to human DMT1.
Application
Research Category
Signaling
Metabolism
Signaling
Metabolism
Research Sub Category
Ion & Transport Channels
Ion Channels & Transporters
Ion & Transport Channels
Ion Channels & Transporters
This Anti-DMT1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of DMT1.
Western Blot Analysis: 2 µg/ml of this antibody detected DMT1 in 50 µg of mouse liver, spleen and duodenum tissue lysate (Schumann, K.,et al. (2010). Journal of Trace Elements in Medicine and Biology 24 (2010) 58–66)
Immunocytochemistry Analysis: A 1:100 dilution of this antibody from a representative lot detected DMT1 in Hela, A431, and N1H-3T3 cells
Immunocytochemistry Analysis: A 1:100 dilution of this antibody from a representative lot detected DMT1 in Hela, A431, and N1H-3T3 cells
Quality
Evaluated by Western Blotting in human placenta tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DMT1 in 10 µg of human placenta tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DMT1 in 10 µg of human placenta tissue lysate.
Target description
~ 62 kDa observed
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Irina Belaya et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Dysregulation of brain iron metabolism is one of the pathological features of aging and Alzheimer's disease (AD), a neurodegenerative disease characterized by progressive memory loss and cognitive impairment. While physical inactivity is one of the risk factors for AD and
Stephanie L Baringer et al.
Journal of biomedical science, 30(1), 36-36 (2023-06-06)
Apo- (iron free) and holo- (iron bound) transferrin (Tf) participate in precise regulation of brain iron uptake at endothelial cells of the blood-brain barrier. Apo-Tf indicates an iron-deficient environment and stimulates iron release, while holo-Tf indicates an iron sufficient environment
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Mutations in several genes involved in the epigenetic regulation of gene expression have been considered risk alterations to different intellectual disability (ID) syndromes associated with features of autism spectrum disorder (ASD). Among them are the pathogenic variants of the lysine-acetyltransferase
Huynh Tan Hop et al.
EMBO reports, 24(9), e55376-e55376 (2023-07-28)
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