ABS72
Anti-WAS protein family homolog 1 (CT) Antibody
from rabbit
Synonym(s):
WAS protein family homolog 1, family with sequence similarity 39, member E, CXYorf1-like protein on chromosome 2, Protein FAM39E
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WASH1(100287171)
General description
Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) is a novel protein belonging to the WASP family, a group of nucleation promoting factors (NFPs) that activate a critical component in endocytosis, Arp 2/3. WASH contains three specific domains: a C-terminal VCA domain, a proline-rich domain, and an N terminal region containing two WASH homolog regions, WHD1 and WHD2. Although WASH has yet to be completely characterized, it is known to function as an NPF at the endosomal surface, where it is involved in the recruitment and subsequent activation of Arp2/3 in order to initiate actin polymerization. Therefore, WASH has a crucial role in endosomal sorting as a mediator of tubule fission resulting from Arp2/3 activation.
Specificity
This antibody recognizes WAS protein family homolog 1 at the C-terminus.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to human WAS protein family homolog 1 at the C-terminus.
Application
Anti-WAS protein family homolog 1 (C-terminus) Antibody detects level of WAS protein family homolog 1 (C-terminus) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected WAS protein family homolog 1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected WAS protein family homolog 1 on 10 µg of HeLa cell lysate.
Target description
~ 60 kDa observed. The calculated molecular weight is 50 kDa
Physical form
Format: Purified
Protein G
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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María Tello-Lafoz et al.
Traffic (Copenhagen, Denmark), 18(8), 491-504 (2017-05-10)
T Lymphocyte recognition of antigens leads to the formation of a highly organized structure termed immune synapse (IS) by analogy with the neuronals synapse. Sorting nexin 27 (SNX27) controls the endosomal traffic of PSD95, Dlg1, ZO-1 (PDZ) domain-interacting proteins, and
Eszter Zavodszky et al.
Nature communications, 5, 3828-3828 (2014-05-14)
Endosomal protein sorting controls the localization of many physiologically important proteins and is linked to several neurodegenerative diseases. VPS35 is a component of the retromer complex, which mediates endosome-to-Golgi retrieval of membrane proteins such as the cation-independent mannose 6-phosphate receptor.
Benjamin J Tyrrell et al.
Pigment cell & melanoma research, 29(5), 559-571 (2016-07-09)
The five-subunit WASH complex generates actin networks that participate in endocytic trafficking, migration and invasion in various cell types. Loss of one of the two subunits WASH or strumpellin in mice is lethal, but little is known about their role
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