ABS2107M
Anti-phospho-CaMKII beta (Ser331)
from rabbit, purified by affinity chromatography
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Calcium/calmodulin-dependent protein kinase type II subunit beta, EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II beta
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
species reactivity (predicted by homology)
human (based on 100% sequence homology)
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
target post-translational modification
phosphorylation (pSer331)
Gene Information
rat ... Camk2B(24245)
General description
Calcium/calmodulin-dependent protein kinase type II subunit beta (UniProt: P08413; also known as EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II subunit beta) is encoded by the Camk2b gene (Gene ID: 24245) in rat. CaM kinase II is one of the most widely studied multi-functional enzyme. It is found in high concentrations in the brain and in some regions it may constitute up to 2% of the total protein content. CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (alpha, beta, gamma, and delta). CaMKII beta contains an auto-inhibitory domain (aa 283-293), a calmodulin-binding domain (aa 291-301) and a unique C-terminal association domain responsible for oligomerization. It is activated Ca2+/calmodulin and binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-287 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity that persists even after withdrawal of calcium. Phosphorylation of Thr-287 locks the kinase into an activated state. CaM Kinase II typically recognizes the phosphorylation site sequence Arg-X-X-Ser/Thr. It prefers arginine in the P3 position, which is reported to make an electrostatic contact with Glu96 in the catalytic core.
Specificity
This rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
Immunogen
A linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
Application
Anti-phospho-CaMKII beta (Ser331), Cat. No. ABS2107, is a highly specific rabbit polyclonal antibody that targets Calcium/calmodulin-dependent protein kinase type II subunit beta (CaMK-II subunit beta) and has been tested for use in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Quality
Evaluated by Western Blotting in HEK293T cells transfected with CaMKII WT or mutant S331A.
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT, but not the mutant S331A.
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT, but not the mutant S331A.
Target description
~60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
Certificates of Analysis (COA)
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