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biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... KIAA1715(80856)
General description
Protein lunapark (UniProt Q9C0E8; also known as Ulnaless) is encoded by the LNP (also known as KIAA1715) gene (Gene ID 80856) in human. The endoplasmic reticulum (ER) consists of a polygonal network of sheets and tubules interconnected by three-way junctions that undergoes continual remodeling through two competing processes. The branching and fusion of tubules lead to the formation of new three-way junctions and polygons, while junction sliding and ring closure lead to polygon loss. Both yeast Lnp1 and mammalian lunapark (mLnp1) are reported to play an important role in the maintenance of ER morphology through junctions stabilization. Study conducted in COS-7 cells demonstrated that junctions with lunapark are less mobile than junctions lacking lunapark. New junctions acquired lunapark tend to remain stable within the ER network, whereas the nascent junctions that fail to acquire lunapark preferentially undergo ring closure.
Specificity
Exprected to react with all four spliced isoforms of human lunapark reported by UniProt (Q9C0E8).
Immunogen
GST-tagged full-length recombinant human Lunapark.
Application
Detect Lunapark using this Anti-Lunapark Antibody validated for use in Western Blotting.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Western Blotting Analysis: The purified antibody was used to detect Lunapark expression in COS-7 cells and confirm siRNA-medidated Lunapark downregulation (Chen, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(2):418-423).
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Lunapark in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Lunapark in 10 µg of HeLa cell lysate.
Target description
~47 kDa observed. 47.74 kDa (isoform 1), 47.09 kDa (isoform 2), 33.01 kDa (isoform 3), and 50.85 kDa (isoform 4) calculated.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hiroaki Kajiho et al.
Scientific reports, 9(1), 13152-13152 (2019-09-13)
Endoplasmic reticulum (ER) tubules connect each other by three-way junctions, resulting in a tubular ER network. Oligomerization of three-way junction protein lunapark (Lnp) is important for its localization and the three-way junction stability. On the other hand, Lnp has an
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