Anti-Hypusine Antibody Antibody

The amino acid Hypusine is a unique amino acid and a critical component of only one cellular protein, the eukaryotic translation initiation factor 5A (eIF-5A, old terminology eIF-4D). Hypusine is formed post-translationally in two enzymatic steps that modify a specific lysine in the precursor protein of eIF-5A. Hypusine is produced soon after the translation of eIF-5A and the modification is essentially irreversible. Hypusine is found in all eukaryotes and archaebacteria but not in eubacteria, and the high conservation of the amino acids surrounding the key lysine point to its importance in eukaryotic biology. The expression of eIF-5A is essential as is the lysine for eIF-5A function. eIF-5A is needed for the initiation of translation and other important processes such as responses to cellular stress, and nucleocytoplasmic shuttling of specific mRNAs. Without an active Hypusine cell proliferation is inhibited, thus Hypusine and its biosynthesis are targets of anti-cancer therapies.

This antibody detects hypusine modification on endogenous eIF5A

Linear peptide corresponding to Human Hypusine.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This Anti-Hypusine Antibody Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of Hypusine.

Western Blotting Analysis: A representative lot of this antibody detected hypusine modification on eIF5A protein in stimulated mouse CD90.2 splenocytes (Colvin, S,. et al, J. Biol. Chem. 2013, 288:36226-36235.)
Western Blotting Analysis: 0.4 µg/mL of this antibody detected hypusine modifications on eIF5A protein in transfected INS-1, INS-1 β and 293T cell lysate (Nishiki, Y,. et al. SpringerPlus 2013, 2:421)
Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected hypusine modifications on eIF5A protein in transfected 293T cells (Nishiki, Y,. et al. SpringerPlus 2013, 2:421)
Immunohistochemistry Analysis: 0.6 µg/mL of this antibody detected hypusine modifications on eIF5A protein in human spleen cells

Evaluated by Western Blotting in transfected HEK293T cell lysate.

Western Blotting Analysis: 0.6 µg/mL of this antibody detected Hypusine in 10 µg of HEK293T transfected with GFP-eIF5A WT cell lysate

~ 45 kDa observed (Nishiki et al. SpringerPlus 2013, 2:421)

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 30% glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Do not store in -80°C.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.