ABS1045
Anti-phospho Angiomotin Antibody (Ser175)
from rabbit
Synonym(s):
Angiomotin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer175)
Gene Information
human ... AMOT(154796)
General description
Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.
Specificity
This antibody only detects p130 isoform.
Immunogen
Epitope: Phosphorylated Ser175
Linear peptide corresponding to human phospho Angiomotin (Ser175).
Application
Anti-phospho Angiomotin Antibody (Ser175) | ABS1045 is an antibody against phospho Angiomotin Antibody for use in Western Blotting.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Western Blotting in HEK293 cell lysate transfected with LATS (Angiomotin Kinase).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho Angiomotin (Ser175) in 10 µg of HEK293 transfected with LATS.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho Angiomotin (Ser175) in 10 µg of HEK293 transfected with LATS.
Target description
~150 kDa observed. The calculated molecular weight is 118 kDa, however phospho Angiomotin (Ser175) has been shown as a ~150 kDa band in western blots (Yi, C., et al. (2011). Cancer Cell. 19(4):527-540).
Physical form
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
T-gel
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Patrizia Brunner et al.
Molecular biology of the cell, 31(2), 118-130 (2019-12-05)
The large isoform of the transmembrane protein angiomotin (AMOT130) controls cell proliferation and migration of many cell types. AMOT130 associates to the actin cytoskeleton and regulates tight-junction maintenance and signaling often via endosomal uptake of polarity proteins at tight junctions.
Phillip H Kang et al.
Molecular biology of the cell, 31(5), 386-396 (2020-01-16)
Mechanical cues regulate the function of a broad range of stem cells in culture and in tissue. For example, soft substrates promote the neuronal differentiation of neural stem cells (NSCs) by suppressing cytoskeletal contractility. However, the mechanisms that link cytoskeletal
Michael Wigerius et al.
The Journal of cell biology, 217(2), 715-730 (2018-01-11)
The actin cytoskeleton is essential for the structural changes in dendritic spines that lead to the formation of new synapses. Although the molecular mechanisms underlying spine formation are well characterized, the events that drive spine maturation during development are largely
Xianle Shi et al.
Development (Cambridge, England), 144(21), 3957-3967 (2017-09-28)
The Hippo pathway modulates the transcriptional activity of Yap to regulate the differentiation of the inner cell mass (ICM) and the trophectoderm (TE) in blastocysts. Yet how Hippo signaling is differentially regulated in ICM and TE cells is poorly understood.
Susana Moleirinho et al.
eLife, 6 (2017-05-04)
The Hippo-YAP pathway is a central regulator of cell contact inhibition, proliferation and death. There are conflicting reports regarding the role of Angiomotin (Amot) in regulating this pathway. While some studies suggest a YAP-inhibitory function other studies indicate Amot is
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