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ABS1017

Sigma-Aldrich

Anti-PHLPP2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

PHLPP2, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PHLPP2(23035)

Related Categories

General description

PH Domain And Leucine Rich Repeat Protein Phosphatase 2 is encoded by the PHLPP2 gene. It is known to catalyze the dephosphorylation of pSer473 of AKT1 and pSer660 of PRKCbeta-II, as well as pSer657 of PRKCalpha.

Immunogen

Epitope: Within PP2C-like Domain
KLH-conjugated linear peptide corresponding within the PP2C-like domain of human PHLPP2.

Application

Anti-PHLPP2 Antibody is an antibody against PHLPP2 for use in Western Blotting and Immunocytochemistry.
Immunocytochemistry Analysis: 2.0 ug/mL from a representative lot detected PHLPP2 in HUVEC cells.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected PHLPP2 in 10 µg of HUVEC cell lysate.

Target description

~150 kDa observed. Predicted to cross-react to all isoforms. Isoform 2 (~107kDa) can possibly be detected at longer exposures/higher concentrations or in certain tissues.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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