Anti-ZNT3 pAb Antibody

Zinc transporter 3 (UniProt P97441; also known as Solute carrier family 30 member 3, ZnT-3) is encoded by the Slc30a3 (also known as Znt3) gene (Gene ID 22784) in murine species. Zinc transporters mediate the transport of zinc ions into intracellular compartments as well as the export of excess zinc out of cells. Disruption of zinc homeostasis is reponsible for many chronic neurodegenerative diseases and acute neural injuries. Excessive and inadequate levels of bioavailable zinc are detrimental to the health of neurons. Two classes of multipass transmembrane Zn transporters are known, including 10 ZnTs and 14 ZIPs, that exhibit opposing functions in mediating zinc homeostasis . These transporters are located on plasma and vesicular membranes with tissue-specific expression pattern. ZnT-3 is an output type Zn transporter expressed in brain, retina, and testis. All ten ZnTs that have been described share similar cellular topology containing 6 transmembrane domains (a.a. 76-96, 106-126, 146-166, 178-198, 236-256, 264-284 of murine ZnT-3), 4 cytoplasmic and 3 vacuolar domains, having both N- and C-terminal ends (a.a. 1-75 and 285-388 of murine ZnT-3) exposed at the cytoplasmic side, except for ZnT-5 which has extra 10 predicted transmembrane domains at the N-terminal end.

This polyclonal antibody specifically detected exogenously expressed murine ZnT-3 with no cross-reactivity toward exogenously expressed murine ZnT-1 or ZnT-2 (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939). This polyclonal antibody specifically detected ZnT-3 expression in wild-type, but not Znt3-knockout, mouse brain tissues (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).

Epitope: C-terminal cytoplasmic tail.

MBP-tagged recombinant mouse ZnT-3 C-terminal fragment.

Anti-ZNT3 pAb Antibody is an antibody against ZNT3 pAb for use in Western Blotting, Immunohistochemistry, Immunofluorescence, Electron Microscopy.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western Blotting Analysis: A representative lot detected an upregulated ZnT-3 level in hippocampus tissue lysates from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309).
Western Blotting Analysis: A representative lot detected ZnT-3 expression in brain lysates from wild-type, but not Znt3-knockout mice, while reduced ZnT-3 level was seen in brain tissues from Znt3+/- mice (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).
Western Blotting Analysis: A representative lot detected exogenously expressed murine ZnT-3 in transfected BHK baby hamster kidney fibroblasts and endogenous ZnT-3 in mouse brain, but not testis or kidney, tissue homogenates. No cross-reactivity was seen toward murine ZnT-1 or ZnT-2 (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939).
Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in the hippocampus inner molecular layer (IML) of 4% paraformaldehyde-/0.025% glutaraldehyde-fixed brain sections from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309).
Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in brain regions of wild-type, but not Znt3-knockout mice, while reduced ZnT-3 immunoreactivity was seen in brain tissues from Znt3+/- mice using 4% paraformaldehyde-fixed frozen brain sections (Cole, T.B., et al. (1999). Proc. Natl. Acad. Sci. U. S. A. 96(4):1716-17121).
Immunohistochemistry Analysis: A representative lot detected ZnT-3 immunoreactivity in the zinc-containing mossy fiber (MF) projections in mouse and monkey hippocampus using 4% paraformaldehyde-fixed frozen brain sections (Wenzel, H.J., et al. (1997). Proc. Natl. Acad. Sci. U. S. A. 94(23):12676-12681).
Immunohistochemistry Analysis: A representative lot immunostained Zn-rich mossy fiber projections emanating from dentate gyrus granule cell neurons as well as projections from hippocampal and cortical neurons in 4% paraformaldehyde-fixed frozen mouse brain sections (Palmiter, R.D., et al. (1996). Proc. Natl. Acad. Sci. U. S. A. 93(25):14934-14939).
Immunofluorescence Analysis: A representative lot immunostained mossy fiber (MF) sprouting in the hippocampus inner molecular layer (IML) by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed free-floating brain sections from pilocarpine-induced epileptic mice with or without medial ganglionic eminence (MGE) progenitor cell grafts (Hunt, R.F., et al. (2013). Nat. Neurosci. 16(6):692-697).
Immunofluorescence Analysis: A representative lot detected ZnT-3 immunoreactivity in Aβ-positive senile plaques (SP) by dual fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed, paraffin-embedded Alzheimer′s diseased human cerebral cortex sections (Zhang, L.H., et al. (2008). Brain Res. Bull. 77(1):55-60).
Electron Microscopy: A representative lot immunostained mossy fiber bouton (MFB) in the hippocampus inner molecular layer (IML) of 4% paraformaldehyde-/0.025% glutaraldehyde-fixed frozen brain sections from mice undergoing spontaneous recurrent seizures (SRS) induction by pilocarpine treatment (Chi, Z.H., et al. (2008). Neurochem. Int. 52(7):1305-1309).
Electron Microscopy: A representative lot detected ZnT-3 immunoreactivity at the terminals of glutamatergic projections in mouse and monkey hippocampus by EM using 4% paraformaldehyde-fixed frozen brain sections (Wenzel, H.J., et al. (1997). Proc. Natl. Acad. Sci. U. S. A. 94(23):12676-12681).

Evaluated by Western Blotting in Alzheimer′s diseased human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ZNT3 in 10 µg of Alzheimer′s diseased human brain tissue lysate.

~45 kDa observed. 41.82 kDa (mouse) and 41.95 kDa (human) calculated.

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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