ABN99
Anti-phospho NR1 (Ser897) Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
glutamate receptor, ionotropic, N-methyl D-aspartate 1, N-methyl-D-aspartate receptor subunit NR1, glutamate [NMDA] receptor subunit zeta 1, N-methyl-D-aspartate receptor channel, subunit zeta-1, glutamate [NMDA] receptor subunit zeta-1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), human (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer897)
Gene Information
human ... GRIN1(2902)
mouse ... Grin1(14810)
rat ... Grin1(24408)
General description
NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
Specificity
This antibody recognizes NR1 when phosphorylated at Ser897.
Immunogen
Epitope: Phosphorylated Ser897
KLH-conjugated linear peptide corresponding to rat NR1 when phosphorylated at Ser897.
Application
Immunohistochemistry Analysis: 1:50 dilution from a previous lot detected NR1 in normal rat hippocampus.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated NR1 from rat brain microsomal RIPA lysate.
Western Blot Analysis: 2 µg/mL from a previous lot detected NR1 in Lambda phosphatase untreated and treated rat brain microsomal tissue lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated NR1 from rat brain microsomal RIPA lysate.
Western Blot Analysis: 2 µg/mL from a previous lot detected NR1 in Lambda phosphatase untreated and treated rat brain microsomal tissue lysate.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-phospho NR1 (Ser897) Antibody is validated for use in WB, IP, IH for the detection of phospho NR1 (Ser897).
Quality
Evaluated by Western Blot in rat brain microsomal tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of rat brain microsomal tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of rat brain microsomal tissue lysate.
Target description
~ 120 kDa
Linkage
Replaces: 06-641
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain microsomal tissue lysate
Rat brain microsomal tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Wenjuan Ru et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 11(1), 182-191 (2015-11-20)
HIV-1 envelope glycoprotein gp120 (gp120) is a major virulence protein implicated in the pathogenesis of HIV-associated neurocognitive disorders (HAND). Although gp120 has been suggested to cause synaptic and neuronal injuries by disrupting NMDA receptor (NMDAR) function, the underlying mechanism is
Lucas V Lima et al.
Pain, 158(9), 1697-1710 (2017-06-18)
Regular physical activity prevents the development of chronic muscle pain through the modulation of central mechanisms that involve rostral ventromedial medulla (RVM). We tested if pharmacological blockade or genetic deletion of mu-opioid receptors in physically active mice modulates excitatory and
Regular physical activity prevents development of chronic pain and activation of central neurons.
Sluka, KA; O'Donnell, JM; Danielson, J; Rasmussen, LA
Journal of Applied Physiology (1985)
Jinseok Yeo et al.
Journal of pain research, 11, 3025-3030 (2018-12-21)
Complex regional pain syndrome type 1 (CRPS I) is an intractable neuropathic pain syndrome. Chronic post-ischemia pain (CPIP) model is an animal model of CRPS I which is produced by ischemia-reperfusion (IR) injury of the hind limb. Dexmedetomidine (DEX) is
Jinseok Yeo et al.
Pain research & management, 2017, 7394626-7394626 (2017-12-07)
The chronic postischemia pain (CPIP) model is an animal model using ischemia/reperfusion injury that mimics the symptoms of complex regional pain syndrome type I. Glutathione (GSH) prevents ischemia/reperfusion injury by scavenging free radicals. We conducted this study to investigate the
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