biological source
guinea pig
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)
General description
ABN90 is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Application
- Immunohistochemistry (Paraffin) Analysis: A 1:5,000 dilution from a representative lot detected NeuN in mouse cerebral cortex and hippocampus brain tissue sections.
- Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortical cells.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
This guinea pig polyclonal Anti-NeuN, Cat. No. ABN90 is tested for use in Western Blotting, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of NeuN.
Quality
Evaluated by Western Blotting in Mouse E16 brain tissue lysate. Western Blotting Analysis: A 1:2,000 Dilution of this antibody detected NeuN in Mouse E16 brain tissue lysate.
Target description
~48 kDa observed
Physical form
Guinea pig polyclonal serum containing 0.05% sodium azide.
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WGK 1
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