ABN446
Anti-Capicua Antibody
from rabbit, purified by affinity chromatography
Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
Protein capicua homolog
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
ox (based on 100% sequence homology), hamster (based on 100% sequence homology), mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CIC(23152)
General description
Protein capicua homolog (CIC) , also known as Capicua Transcriptional Repressor, Capicua Homolog, and encoded by the gene CIC, is a transcriptional repressor which plays a role in development of the central nervous system (CNS). CIC is a new subfamily of the HMG-box superfamily and the gene′s expression is predominantly restricted to immature granule cells in the cerebellum, hippocampus and olfactory bulb in the CNS, and thus the gene is implicated in CNS development, in particular in granule cell development. CIC appears to be central to cerebellar development and ErbB signaling as well. Recent research has demonstrated that CIC is highly expressed in certain cancers including medulloblastomas, but also the loss of expression may serve as a biomarker for other cancer types such as oligodendrogliomas.
Immunogen
Epitope: Near N-terminal
KLH-conjugated linear peptide corresponding to human Capicua near the N-terminal.
Application
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Capicua in human cerebral cortex tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Capicua Antibody is validated for use in Western Blotting for the detection of Capicua.
Quality
Evaluated by Western Blotting in human cerebellum tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Capicua in 10 µg of human cerebellum tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Capicua in 10 µg of human cerebellum tissue lysate.
Target description
~160 kDa observed. Long isoform can be detected at ~250kDa with longer exposure, in some lysates (Kim, E., et al. (2013). Genea Dev. 27:590-595).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Shih-Chiang Huang et al.
The American journal of surgical pathology, 40(5), 645-655 (2016-01-07)
Angiosarcoma (AS) is a rare sarcoma subtype showing considerable clinicopathologic and genetic heterogeneity. Most radiation-induced AS show MYC gene amplifications, with a subset of cases harboring KDR, PTPRB, and PLCG1 mutations. Despite recent advances, the genetic abnormalities of most primary
Severa Bunda et al.
Nature communications, 10(1), 661-661 (2019-02-10)
Capicua (CIC) is a transcriptional repressor that counteracts activation of genes downstream of receptor tyrosine kinase (RTK)/Ras/ERK signaling. It is well-established that tumorigenesis, especially in glioblastoma (GBM), is attributed to hyperactive RTK/Ras/ERK signaling. While CIC is mutated in other tumors
Severa Bunda et al.
Molecular cancer research : MCR, 18(5), 774-786 (2020-02-08)
Capicua (CIC) is a transcriptional repressor that counteracts activation of genes in response to receptor tyrosine kinase (RTK)/Ras/ERK signaling. Following activation of RTK, ERK enters the nucleus and serine-phosphorylates CIC, releasing it from its targets to permit gene expression. We
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service