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ABN438

Sigma-Aldrich

Anti-ELFN1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Protein ELFN1, Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1, Protein phosphatase 1 regulatory subunit 28
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

300

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

hamster (based on 100% sequence homology), monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), primate (based on 100% sequence homology), baboon (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_001122108

UniProt accession no.

P0C7U0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ELFN1(392617)

General description

ELFN1 is a postsynaptic protein that regulates circuit dynamics in the CNS by modulating the temporal dynamics of interneuron recruitment. ELFN1 is specifically expressed in excitatory synapses onto orens-lacunosum (OLM) interneurons and acts as a regulator of presynaptic release in directing of the pyramidal-OLM synapses. ELFN1 localizes to excitatory synapses on somatostatin containing OLM interneurons at the plasma membrane, and ELFN1 protein, expressed post-synaptically, can direct the elaboration of specific electrochemical properties of synapses between particular cell types in the hippocampus. ELFN1’s expression is found in distinct subsets of interneurons of the hippocampus and cortex, and also in discrete subsets of cells in the habenula, septum, globus pallidus, dorsal subiculum, amygdala and several other brain regions in the mouse. The protein is also expressed in diverse cell types, including local GABAergic interneurons as well as long-range projecting GABAergic and glutamatergic neurons.

Immunogen

Epitope: Near N-terminal, extracellular domain
KLH-conjugated linear peptide corresponding to human ELFN1 near the N-terminal, extracellular domain.

Application

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ELFN1 in human thalamus and rat cerebral cortex tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-ELFN1 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of ELFN1.

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ELFN1 in 10 µg of U2OS cell lysate.

Target description

~100 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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