ABN306
Anti-MANF Antibody
from rabbit, purified by affinity chromatography
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Synonym(s):
Mesencephalic astrocyte-derived neurotrophic factor, arginine-rich mutatedin early stage tumor, ARMET, ARP
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MANF(7873)
General description
MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson’s disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.
Specificity
This antibody does not cross-react with CDNF.
Immunogen
KLH-conjugated linear peptide corresponding to human MANF.
Application
Detect the MANF protein using this Anti-MANF validated for use in WB & IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected MANF in human midbrain and rat brain tissues.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Quality
Evaluated by Western Blot in PC12 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MANF in 15 µg/mL of PC12 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MANF in 15 µg/mL of PC12 cell lysate.
Target description
~18 kDa observed. The calculated molecular weight is 21 kDa, however MANF has been shown as an ~18 kDa band in western blots. (Mizobuchi, N., et al. (2007). Cell Structure and Function. 32:41-50.). Uncharacterized bands may appear at ~25 kDa and ~100 kDa in some lysates.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
PC12 cell lysate
PC12 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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