Anti-Cullin-5 (CUL5) Antibody

CUL-5/VACM-1 (cullin-5, vasopressin-activated calcium mobilizing receptor 1) is one component of the ECS (elongin B/C-cul2/cul5-SOCS box protein) complex that forms an E3 ubiquitin ligase that covalently binds ubiquitin to proteins targeted for degradation via the proteasome. CUL-5 is ubiquitously expressed in the central nervous system and in many peripheral organs. Human immunodeficiency virus-1 (HIV-1) protein Vif forms a Skp1-cullin-F-box (SCF)-like complex with CUL-5/VACM-1 and Elongins B and C. The ability of Vif to suppress the antiviral activity of APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is specifically dependent on CUL-5-SCF function, as it allows Vif to interact with APOBEC3G and induce its ubiquitination and degradation. The CUL-5-SCF pathway used by Vif is thus a potential target for antiviral development. CUL-5/VACM-1 inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways. In human tissues expression in kidney, brain, heart, placenta, and skeletal muscle is observed. CUL-5/VACM-1 is also expressed in both breast epithelial cells and breast cancer cells. Decreased expression of CUL-5/VACM-1 may play a role in breast tumorigenesis. Further evidence for this finding is observed in T47D breast cancer cells in which overexpression of CUL-5/VACM-1 significantly attenuates cellular proliferation and MAPK phosphorylation when compared to control cells.

Other homologies: Feline (93% sequence homology) and Mouse and Rat (87% sequence homology).

This antibody recognizes Cullin-5 near the N-Terminus.

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to human Cullin-5 near the Near N-Terminus.

Detect Cullin using this rabbit polyclonal antibody, Anti-Cullin-5 (CUL5) Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cullin-5 in human placenta and human testis tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cullin-5 in 10 µg of human heart tissue lysate.

~90 kDa observed. Neddylation of Cullin-5 at a.a. 724 with NEDD8 results in a 10 kDa increase in molecular weight (Hori. T., et al. (1999). Oncogene. 18(48):6829-6834).

Replaces: AB10622

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human heart tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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