All Photos(2)
IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... PCSK1N(27344)
mouse ... Pcsk1N(30052)
General description
ProSAAS (UniProt: Q9QXV0; also known as IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS) is encoded by the Pcsk1n gene (Gene ID: 30052) in murine species. ProSAAS is a small secretory protein that is primarily expressed in neural and neuroendocrine tissues and acts as an anti-aggregant chaperone. Its presence appears to be restricted only to vertebrates. Mammalian proSAAS does not undergo either glycosylation or phosphorylation, but undergoes proteolytic cleavage at several pairs of basic residues that leaves its core region intact. ProSAAS is known to interact with proprotein convertase subtilisin/kexin type 1 (PCSK1) via the C-terminal inhibitory domain (aa 219-258). In the neural tissues proSAAS exhibits neuroprotective effects and is shown to block alpha-synuclein-induced cytotoxicity in cultures of dopaminergic neurons and in SH-SY5Y cells. In vitro studies have shown that proSAAS can also prevent the fibrillation of amyloid beta 1-42 peptide in a dose-dependent manner and residues 97-180 are sufficient for its anti-aggregation function against amyloid peptides. Inclusion of recombinant proSAAS in the medium of Neuro2a cells is shown to block the neurocytotoxic effect of amyloid peptide 1-42.
Specificity
This rabbit polyclonal antibody detects proSAAS in human, mouse, and rat cells. It targets an epitope within the N-terminal half.
Immunogen
His-tagged recombinant fragment corresponding to first 180 amino acids from murine ProSAAS.
Application
Anti-proSAAS Antibody, Cat. No. ABN2268, is a highly specific rabbit polyclonal antibody that targets ProSAAS and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected proSAAS in AtT-20 cells. (Courtesy of Dr. Iris Lindberg @ University of Maryland).
Immunofluorescence Analysis: A representative lot detected proSAAS in Immunofluorescence applications (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15; Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Guest, P.C., et. al. (2002). J Endocrinol. 175(3):637-47).
Western Blotting Analysis: A representative lot detected proSAAS in A immunoprecipitated from mouse brain lysates (Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30). Notes: Treating blots with 1% glutaraldehyde in PBS for 30 min prior to blocking significantly increased the proSAAS signal and reduces the background.
Immunocytochemistry Analysis: A representative lot detected proSAAS in SH-SY5Y cells (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15).
Immunofluorescence Analysis: A representative lot detected proSAAS in Immunofluorescence applications (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15; Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Guest, P.C., et. al. (2002). J Endocrinol. 175(3):637-47).
Western Blotting Analysis: A representative lot detected proSAAS in A immunoprecipitated from mouse brain lysates (Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30). Notes: Treating blots with 1% glutaraldehyde in PBS for 30 min prior to blocking significantly increased the proSAAS signal and reduces the background.
Immunocytochemistry Analysis: A representative lot detected proSAAS in SH-SY5Y cells (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15).
Quality
Evaluated by Western Blotting in His-tagged mouse proSAAS recombinant protein.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged mouse proSAAS recombinant protein. Note: For most biological samples a range of 1:1000 for Western blotting and 1:500 for IHC is recommended.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged mouse proSAAS recombinant protein. Note: For most biological samples a range of 1:1000 for Western blotting and 1:500 for IHC is recommended.
Target description
~19 kDa observed; 27.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Other Notes
Concentration: Please refer to lot specific datasheet.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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