Anti-Aβ-42 Antibody, oligomeric (VIA)

Amyloid beta A4 protein (UniProt P05067; also known as ABPP, Alzheimer disease amyloid protein, Amyloid precursor protein, APP, APPI, Cerebral vascular amyloid peptide, CVAP, PN-II, PreA4, Protease nexin-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID 351) in human. Amyloid precursor protein (APP) is initially produced with a signal peptide sequence (a.a. 1-17), the removal of which yields the mature protein with a large extracellular portion (a.a. 18-699), followed by a transmembrane segment (a.a. 700-723) and a cytoplasmic (a.a. 724-770) tail. APP can be further processed by the α-, β-, and γ-secretases in two alternative processing pathways. In the non-amyloidogenic pathway, APP is first cleaved by the plasma membrane-localized α-secretase to generate an N-terminal extracellular sAPPα fragment (a.a. 18-687) and a membrane-bound C-terminal fragment C83 (CTFα), which can be further cleaved by γ-secretase to produce a non-toxic small peptide p3 and a cytoplasmic APP intracellular domain (AICD). In the amyloidogenic pathway, APP undergoes β-cleavage in BACE-1 (β-site APP-cleaving enzyme)-enriched endosomes to generate an N-terminal extracellular sAPPβ fragment (a.a. 18-671) and a membrane-bound C-terminal fragment C99 (CTFβ). Subsequent cleavage of C99 by γ-secretase releases the amyloid β peptides, Aβ1-42 (672-713) & Aβ1-40 (672-711), and AICD. Aβ accumulation in the cortical and hippocampal regions of the brain is a major pathological feature of Alzheimer′s disease (AD). Growing evidences implicate soluble oligomers as the more toxic species and the extent of oligomer formation and assembly correlates better with disease progression and cognitive dysfunction. These Aβ oligomers are able to induce other aggregation-prone proteins, including α-synuclein (α-syn), prion protein (PrP), and TDP-43, to assume oligomeric conformations. These proteins can then seed tau aggregation, resulting in neurodegeneration.

This polyclonal antiserum (VIA) recognizes a distinct epitope specific to Aβ-42 oligomers. VIA does not recognize classic amyloid plaques composed of fibrillar Aβ or Aβ-40 (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Aggregated synthetic hexapeptide VIAVIA composed of two copies of Aβ-42 C-terminal end 3-amino acid sequence (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Anti-Aβ-42, oligomeric (VIA), Cat. No. ABN1650, is a highly specific rabbit polyclonal antibody that targets oligomeric amyloid beta 42 and has been tested in Dot Blot, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected oligomeric Aβ-42, but not oligomeric Tau or large amyloid plaques in Alzheimer′s diseased (AD) human brain cryosections (Courtesy of Riddhi U Bodani, Kayed lab, Univ of Texas Medical Branch, Galveston, U.S.A.).

Immunohistochemistry Analysis: A 1:150 dilution from a representative lot detected oligomeric Aβ-42 immunoreactivity in Alzheimer′s diseased (AD) human cortex cryosections (Courtesy of Riddhi U Bodani, Kayed lab, Univ of Texas Medical Branch, Galveston, U.S.A.).

Dot Blot Analysis: A representative lot detected recombinant Aβ-42, but not Aβ-40, oligomers (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Immunofluorescence Analysis: A representative lot immunostained both prefibrillar and fibrillar Aβ-42 oligomers in Alzheimer′s diseased (AD) human brain (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Immunofluorescence Analysis: A representative lot detected oligomeric Aβ-42, but not amyloid plaques in coronal sections of Tg2576AD mice harboring human Swedish mutation APP (APPK670/671L) transgene (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Immunohistochemistry Analysis: A representative lot immunostained intracellular perinuclear Aβ-42 aggregates, but not amyloid plaques in Alzheimer′s diseased (AD) human brain (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Immunohistochemistry Analysis: A representative lot detected oligomeric Aβ-42 immunoreactivity in the hippocampus CA1 region of Tg2576AD mice harboring human Swedish mutation APP (APPK670/671L) transgene (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Western Blotting Analysis: A representative lot detected oligomeric A -42, but not monomeric Aβ-42, monomeric or oligomeric Aβ-40 (Bodani, R.U., et al. (2015). ACS Chem. Neurosci. 6(12):1981-1989).

Note: Both purified antibody (Cat. No. ABN1665) and unpurified antiserum (Cat. No. ABN1650) are suitable for Dot blot, immunofluorescence, immunohistochemistry, and Western blotting applications. However, we recommend using only the purified antibody for neutralization studies.

Research Category
Neuroscience

Evaluated by Dot Bot analysis of Aβ proteins.

Dot Blot Analysis: A 1:150 dilution of this antiserum detected recombinant Aβ-42, but not Aβ-40, oligomer.

Variable depending on the sizes of the Aβ-42 oligmers.

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.