All Photos(2)
Synonym(s):
Interferon regulatory factor 7|IRF-7|IRF7
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
clone
polyclonal
species reactivity
mouse
technique(s)
ChIP: suitable
western blot: suitable
target post-translational modification
unmodified
General description
Mouse Interferon regulatory factor 7 or IRF-7 (UniProt P70434) is encoded by the murine irf7 gene (Entrez Gene ID 54123). IFN-γ-induced gene activations are mediated by both signal transducers and activators of transcription (STATs) and interferon regulatory factors (IRFs). STAT1 dimer, for example, stimulates immediate transcription by binding IFN-γ-activated site (GAS) sequence in the promoter region of target genes. While a delayed, secondary transcription activation is observed among IFN-γ target genes containing an IFN regulatory factors (IRF) binding site within the interferon-stimulated response element (ISRE) sequence. Irf7 transcription is shown to be activated by STAT1 and IRF9 in response to type II interferon (IFN) IFN-γ stimulation in MEF cultures. The expressed IRF7 protein then further cooperates with STAT1 and IRF1 to stimulate the expression of a subset of IFN-γ-induced STAT1 target genes. Cat. No. ABF130, Anti-IRF7 is a rabbit polyclonal antiserum raised against recombinant GST fusion protein encompassing murine IRF7 C-terminal half sequence. This antiserum specifically detects INF-gamma-induced IRF7 expression and chromatin association in Wt, but not Irf7-/- MEF by Western blotting and ChIP application, respectively. Murine IRF7 has a calculated M.W. of 51.2 kDa, but migrates with an apparent M.W. of ~60 kDa by SDS-PAGE.
Immunogen
GST-tagged recombinant protein corresponding to the C-terminal of mouse IRF7.
Application
Anti-IRF7 Antibody is an antibody against IRF7 for use in Western Blotting, Chromatin Immunoprecipitation .
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated IRF7-associated DNA fragments using chromatin prepared from infected (12 hours with Vesicular Stomatitis Virus/VSV) or uninfected MEFs cells (Thomas Decker, Max F. Perutz Lab
Quality
Evaluated by Western Blotting in MEF-1 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected IRF7 in 10 ug of MEF-1 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected IRF7 in 10 ug of MEF-1 cell lysate.
Other Notes
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Matthias Farlik et al.
Molecular and cellular biology, 32(6), 1032-1043 (2012-01-19)
Signal transducers and activators of transcription (STATs) and interferon regulatory factors (IRFs) share common target genes. Here we show that the Irf7 gene is regulated by transcription factors STAT1 and IRF9 in response to the type II interferon (IFN) IFN-γ.
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