ABF1159
Anti-DEFA1
from rabbit, purified by affinity chromatography
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HP 1-56, Neutrophil defensin HP 1-56 chain
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
rhesus macaque (based on 100% sequence homology)
technique(s)
flow cytometry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... DEFA1(1667)
Related Categories
General description
The human DEFA1 gene (also known as DEF1, DEFA2, MRS, DEFA1B; Gene ID 1667) encodes a 94-amino acid precursor protein (UniProt P59665) with an N-terminal signal peptide (a.a. 1-19) and propeptide (a.a. 20-38) sequence. Posttranslational processing by proteolytic cleavages generates three neutrophil granule peptides, HP 1-56 (a.a. 39-94), neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94), and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94). These peptides exhibit several biological activities including the non-oxidative destruction of ingested microorganisms, the inhibition of adrenocorticotropin-stimulated glucocorticoids synthesis, monocyte chemotaxis, the non-cytolytic inhibition of thymidine incorporation in HL-60 promyelocyte-like cells and the stimulation of nifedipine-sensitive calcium channels. The richest source of HP1-56 relative to HP-1 is found in fetal lung tissue. In addition, HP1-56 is found in the HL-60 promyelocytic cell line and in some leukemias, but not in normal mature circulating neutrophils.
Specificity
This rabbit polyclonal antibody targets a region present in prepro-, pro-, and mature HP 1-56 (a.a. 1-94, 20-94, and 39-94, respectively). Target region is absent from neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94) and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94).
Immunogen
KLH-conjuated linear peptide corresponding to an internal sequence of human HP 1-56.
Application
Flow Cytometry Analysis: 0.1 µL from a representative lot detected HP 1-56 immunoreactivity in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 human promyelocytic leukemia cells.
Research Category
Inflammation & Immunology
Inflammation & Immunology
This rabbit polyclonal Anti-DEFA1 Antibody, Cat. No. ABF1159 is validated for use in Flow Cytometry and Western Blotting for the detection of HP 1-56.
Quality
Evaluated by Western Blotting in HL-60 cell lysate.
Western Blotting Analysis: A 1:5000 dilution of this antibody detected HP 1-56 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.
Western Blotting Analysis: A 1:5000 dilution of this antibody detected HP 1-56 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.
Target description
~10 kDa observed. 10.20/8.238/6.306 kDa (prepro-/pro-/mature HP 1-56) calculted. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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